Interphase Chromosomes in Replicative Senescence: Chromosome Positioning as a Senescence Biomarker and the Lack of Nuclear Motor-Driven Chromosome Repositioning in Senescent Cells

This study demonstrates, and confirms, that chromosome territory positioning is altered in primary senescent human dermal fibroblasts (HDFs). The pattern very similar to found HDFs made quiescent either by serum starvation or confluence; but not completely. A few chromosomes are different locations. One particular stands out, 10, which located an intermediate location young proliferating HDFs, at the nuclear periphery cells opposing of interior HDFs. We have previously demonstrated individual territories can be actively rapidly relocated, with 15 min, after removal from culture media. These relocations require motor activity through presence myosin 1β (NM1β). now also demonstrate rapid movement heat-shock 42°C. Others shown heat shock genes relocated using protein via actin NM1β ( Khanna et al., 2014 ; Pradhan 2020 ). However, this current reveals, no longer expected locations upon these two types stimuli. coincides a entirely organisation distribution within